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林翰佳 教授

姓     名:林翰佳 老師實驗室
英 文 姓 名:Han-Jia Lin
研 究 專 長:藻類生技、合成生物學、奈米生技
個人實驗室:https://www.hanjialab.org/
電 子 信 箱:hanjia@ntou.edu.tw
研究室位置:人社院地下室 B101
研究室電話:2462-2192 ext. 5524
 
主要授課課程:
  • 普通生物學
  • 生物化學
  • 生技操作
 
學經歷:
  • 學士:台灣大學 農業化學
  • 碩士:台灣大學 生化科學
  • 博士:台灣大學 生化科學
  • 博士後研究:日本大阪大學遺傳情報中心
  • 博士後研究:中研院生化所
  • 台灣多胺學會 理事長
  • 國際多胺研究基金會 科學顧問
 
研究項目簡介:
  • 矽藻分子遺傳:本實驗室是台灣第一個建立矽藻基因改造的團隊,目前仍持續開發各種矽藻分子遺傳工具,作為未來在矽藻基礎生理研究以及產業利用的基礎。
  • 特殊多胺類代謝機制與應用:研究藻類中特殊多胺的合成酵素,並開發以合成生物學大量生產這些多胺類的應用技術。
  • 分子草藥奈米技術與水產應用:利用奈米材料、生化代謝以及食品加工的跨領域技術,開發出全世界第一的分子草藥,可有效替代抗生素。在水產養殖動物上已經證實可保護動物健康,解決抗生素濫用的問題。未來也有機會應用於禽畜, 寵物, 美妝, 以及農業等其他相關領域。
  • 分子檢測技術開發:開發新穎酵素與試劑,與廠商合作,組成疾病檢測國家隊,目前在水產、獸醫以及人類遺傳檢測有具體成果。
  1. Shou-Kuan Tsai, Cheng-Hsin Shih, Hui-Wen Chang, Kuang-Huan Teng, Wei-En Hsu, Han-Jia Lin, Han-You Lin, Ching-Huei Huang, Hui-Wen Chen and Lih- Chiann Wang (2020, Jun): Replication of a Dog-Origin H6N1 Influenza Virus in Cell Culture and Mice. Viruses, 12, 704. DOI:10.3390/v12070704
  2. Hung-Yun Lin, Wei-Yu Yeh, Sheng-Fang Tsai, Kuo-Ping Chiang, John Han-You Lin, Che-Chia Tsao* and Han-Jia Lin* (2020, May): Biological protective effects against Vibrio infections in grouper larvae using the Strombidium sp. NTOU1, a marine ciliate amenable for scaled-up culture and with an excellent bacteriovorous ability. Frontiers in Marine Science, 7, 373. DOI:10.3389/fmars.2020.00373
  3. Huai-ting Huang, Han-Jia Lin, Hui-Ju Huang, Chih-ching Huang, John Han-You Lin and Li-Li chen (2020, Apr). Synthesis and evaluation of polyamine carbon quantum dots (cQDs) in Litopenaeus vannamei as a therapeutic agent against WSSV. Scientific Reports, 10:7343. DOI:10.1038/s41598-020-64325-5
  4. Shou-Kuan Tsai, Chen-Chih Chen, Han-Jia Lin, Han-You Lin, Ting-Tzu Chen, and Lih-Chiann Wang (2020, Mar). Combination of multiplex reverse transcription recombinase polymerase amplification assay and capillary electrophoresis provides high sensitive and high-throughput simultaneous detection of avian influenza virus subtypes. Journal of Veterinary Science , 21, e24. (SCI). DOI:10.4142/jvs.2020.21.e24 .
  5. Hong-JyuanJian, JiantaoYu, Yu-Jia Li, Binesh Unnikrishnan Yu-Fen Huang, Li- Jyuan Luo, David Hui-Kang Ma, Scott G. Harroun, Huan-Tsung Chang, Han-Jia Lin, Jui-Yang Lai, Chih-Ching Huang (2019, Dec). Highly adhesive carbon quantum dots from biogenic amines for prevention of biofilm formation. Chemical Engineering Journal DOI:10.1016/j.cej.2019.123913.
  6. Chin-Jung Lin, Lung Chang, Han-Wei Chu, Han-Jia Lin, Pei-Ching Chang, Robert Y. L. Wang,* Binesh Unnikrishnan, Ju-Yi Mao, Shiow-Yi Chen,* and Chih-Ching Huang* (2019, Aug). High Amplification of the Antiviral Activity of Curcumin through Transformation into Carbon Quantum Dots . small, 15(41), 1902641. DOI:10.1002/smll.201902641
  7. Ju-Yi Mao, Fu-Yin Lin, Han-Wei Chu, Scott G. Harroun, Jui-Yang Lai, Han-Jia Lin*, Chih-Ching Huang*: In situ synthesis of core-shell carbon nanowires as a potent targeted anticoagulant. Journal of Colloid and Interface Science 05/2019; 552., DOI:10.1016/j.jcis.2019.05.086
  8. A. Venerando, M. Magro, D. Baratella, J. Ugolotti, S. Zanin, O. Malina, R. Zboril, H. Lin, F. Vianello. Biotechnological applications of nanostructured hybrids of polyamine carbon quantum dots and iron oxide nanoparticles. Amino Acids 04/2019 https://doi.org/10.1007/s00726-019-02721-6
  9. Hung-Yun Lin, Han-Jia Lin*: Polyamines in Microalgae: Something Borrowed, Something New. Marine Drugs 01/2019; 17(1):1.,http://DOI:10.3390/md17010001
  10. Tun-Wen Pai, Kuan-Hung Li, Cing-Han Yang, Chin-Hwa Hu, Han-Jia Lin, Wen-Der Wang, Yet-Ran Chen: Multiple model species selection for transcriptomics analysis of non-model organisms. BMC Bioinformatics 08/2018; 19(S9):137-150., DOI:10.1186/s12859-018-2278-z
  11. Po-Chih Kuo, Chia-Wen Lien, Ju-Yi Mao, Binesh Unnikrishnan, Huan-Tsung Chang, Han-Jia Lin*, Chih-Ching Huang*: Detection of urinary spermine by using silver-gold/silver chloride nanozymes. Analytica Chimica Acta 06/2018; 1009., DOI:10.1016/j.aca.2018.01.018
  12. Anisha Anand, Binesh Unnikrishnan, J.-Y. Mao, H.-J. Lin*, C.-C. Huang*: Graphene-based nanofiltration membranes for improving salt rejection, water flux and antifouling–A review. Desalination 03/2018; 429:119-133., DOI:10.1016/j.desal.2017.12.012
  13. Ju-Yi Mao, Han-Wei Li, Shih-Chun Wei, Scott G. Harroun, Ming-Ying Lee, Hung-Yun Lin, Chih-Yu Chung, Chun-Hua Hsu, Yet-Ran Chen, Han-Jia Lin*, Chih-Ching Huang*: DNA Modulates the Interaction of Genetically Engineered DNA-Binding Proteins and Gold Nanoparticles: Diagnosis of High-Risk HPV Infection. ACS Applied Materials & Interfaces 12/2017; 9(51)., DOI:10.1021/acsami.7b13873
  14. Scott G. Harroun, Jui-Yang Lai, Chih-Ching Huang, Shou-Kuan Tsai, Han-Jia Lin*Reborn from the Ashes: Turning Organic Molecules to Antimicrobial Carbon Quantum Dots. 09/2017; 3(11)., DOI:10.1021/acsinfecdis.7b00150
  15. Hong-Jyuan Jian, Ren-Siang Wu, Tzu-Yu Lin, Yu-Jia Li, Han-Jia Lin, Scott G. Harroun, Jui-Yang Lai, Chih-Ching Huang: Super-Cationic Carbon Quantum Dots Synthesized from Spermidine as an Eye Drop Formulation for Topical Treatment of Bacterial Keratitis. ACS Nano 07/2017; 11(7)., DOI:10.1021/acsnano.7b01023
  16. Hung-Yun Lin, Shao-Chieh Yen, Po-Chih Kuo, Chih-Yu Chung, Kuan-Lin Yeh, Ching-Huei Huang, Jeng Chang, Han-Jia Lin*: Alkaline phosphatase promoter as an efficient driving element for exogenic recombinant in the marine diatom Phaeodactylum tricornutum. Algal Research 04/2017; 23:58-65., DOI:10.1016/j.algal.2017.01.007
  17. Pei-Xin Lai, Chung-Wein Chen, Shih-Chun Wei, Tzu-Yu Lin, Hong-Jyuan Jian, Irving Po-Jung Lai, Ju-Yi Mao, Pang-Hung Hsu, Han-Jia Lin, Wen-Shyong Tzou, Shiow-Yi Chen, Scott G. Harroun, Jui-Yang Lai, Chih-Ching Huang: Ultrastrong Trapping of VEGF by Graphene Oxide: Anti-angiogenesis Application. Biomaterials 09/2016; 109., DOI:10.1016/j.biomaterials.2016.09.005
  18. Yu-Jia Li, Scott G. Harroun, Yu-Chia Su, Chun-Fang Huang, Binesh Unnikrishnan, Han-Jia Lin*, Chia-Hua Lin, Chih-Ching Huang*: Synthesis of Self-Assembled Spermidine-Carbon Quantum Dots Effective against Multidrug-Resistant Bacteria. Advanced Healthcare Materials 07/2016; 5(19)., DOI:10.1002/adhm.201600297
  19. Chi-Fu Chou, Han-Jia Lin, Chih-Yu Tsai, Tzung-Yi Lin, Hsin-Yu Chung, Chia-Yin Chiang, Chung-Hao Li, Tun-Wen Pai, Chin-Hwa Hu, Wen-Shyong Tzou: Hypoxia-inducible factor 2-alpha participates in the regulation of intersegmental vessels angiogenesis and neuromast deposition. Journal of Marine Science and Technology 04/2016; 24(2):347-358., DOI:10.6119/JMST-015-0629-1
  20. San-Shan Huang, Shih-Chun Wei, Huan-Tsung Chang, Han-Jia Lin, Chih-Ching Huang: Gold nanoparticles modified with self-assembled hybrid monolayer of triblock aptamers as a photoreversible anticoagulant. Journal of Controlled Release 11/2015; 221., DOI:10.1016/j.jconrel.2015.11.028
  21. Han-Jia Lin, Yu-Jia Li, Chih-Ching Huang: Use of polyamine to synthesize fluorescent carbon quantum dots as bactericide and transfection reagents. Amino Acids 08/2015; 47(8):1670-1670.
  22. Wei-Jane Chiu, Tsung-Kai Ling, Hai-Pang Chiang, Han-Jia Lin, Chih-Ching Huang: Monitoring Cluster Ions Derived from Aptamer-Modified Gold Nanofilms under Laser Desorption/Ionization for the Detection of Circulating Tumor Cells. ACS Applied Materials & Interfaces 04/2015; 7(16)., DOI:10.1021/acsami.5b00731
  23. Ka-Lai Pang, Han-Jia Lin, Hung-Yun Lin, Yu-Fen Huang, Yi-Min Chen: Production of Arachidonic and Eicosapentaenoic Acids by the Marine Oomycete Halophytophthora. Marine Biotechnology 08/2014; 17(2)., DOI:10.1007/s10126-014-9600-1
  24. Tzung-Yi Lin, Chi-Fu Chou, Hsin-Yu Chung, Chia-Yin Chiang, Chung-Hao Li, Jen-Leih Wu, Han-Jia Lin, Tun-Wen Pai, Chin-Hwa Hu, Wen-Shyong Tzou: Hypoxia-Inducible Factor 2 Alpha Is Essential for Hepatic Outgrowth and Functions via the Regulation of leg1 Transcription in the Zebrafish Embryo. PLoS ONE 07/2014; 9(7):e101980., DOI:10.1371/journal.pone.0101980
  25. Wen-Shyong Tzou, Ying Chu, Tzung-Yi Lin, Chin-Hwa Hu, Tun-Wen Pai, Hsin-Fu Liu, Han-Jia Lin, Ildeofonso Cases, Ana Rojas, Mayka Sanchez, Zong-Ye You, Ming-Wei Hsu: Molecular Evolution of Multiple-Level Control of Heme Biosynthesis Pathway in Animal Kingdom. PLoS ONE 01/2014; 9(1):e86718., DOI:10.1371/journal.pone.0086718
  26. Yen-Chun Shiang, Chung-Mao Ou, Shih-Ju Chen, Ting-Yu Ou, Han-Jia Lin*, Chih-Ching Huang, Huan-Tsung Chang*: Highly efficient inhibition of human immunodeficiency virus type 1 reverse transcriptase by aptamers functionalized gold nanoparticles. Nanoscale 02/2013; 5(7)., DOI:10.1039/c3nr33403a
  27. Hung-Yun Lin, Chi-Yu Shih, Hung-Chun Liu, Jeng Chang, Ying-Lan Chen, Yet-Ran Chen, Han-Tso Lin, Yu-Yung Chang, Chun-Hua Hsu, Han-Jia Lin*: Identification and Characterization of an Extracellular Alkaline Phosphatase in the Marine Diatom Phaeodactylum tricornutum. Marine Biotechnology 01/2013; 15(4)., DOI:10.1007/s10126-013-9494-3
  28. Yi-Chin Lien, Ting-Yu Ou, Yu-Tzu Lin, Po-Chih Kuo, Han-Jia Lin*: Duplication and Diversification of the Spermidine/Spermine N-acetyltransferase 1 Genes in Zebrafish. PLoS ONE 01/2013; 8(1):e54017., DOI:10.1371/journal.pone.0054017
  29. Li-Yi Chen, Chih-Ching Huang, Wei-Yu Chen, Han-Jia Lin, Huan-Tsung Chang: Using photoluminescent gold nanodots to detect hemoglobin in diluted blood samples. Biosensors & Bioelectronics 12/2012; 43C(1):38-44., DOI:10.1016/j.bios.2012.11.034
  30. Huan-Chin Tseng, Jyh-Bing Tang, P S Sudhakar Gandhi, Ching-Wei Luo, Chung-Mao Ou, Chia-Jen Tseng, Han-Jia Lin*, Yee-Hsiung Chen: Mutual adaptation between mouse transglutaminase 4 and its native substrates in the formation of copulatory plug. Amino Acids 08/2011; 42(2-3):951-60., DOI:10.1007/s00726-011-1009-9
  31. Han-Jia Lin*, Yi-Chin Lien, Chun-Hua Hsu: A high-throughput colorimetric assay to characterize the enzyme kinetic and cellular activity of spermidine/spermine N1-acetyltransferase 1. Analytical Biochemistry 12/2010; 407(2):226-32., DOI:10.1016/j.ab.2010.07.036
  32. Chao-Jung Chen, Mei-Chun Tseng, Han-Jia Lin, Ting-Wei Lin, Yet-Ran Chen: Visual Indicator for Surfactant Abundance in MS-Based Membrane and General Proteomics Applications. Analytical Chemistry 10/2010; 82(19):8283-90., DOI:10.1021/ac1017937
  33. Hui-Chung Wu, Tzu-Ning Chen, Shao-Hsuan Kao, Hao-Ai Shui, Wei-Jung Chen, Han-Jia Lin, Han-Min Chen: Isoelectric Focusing Management: An Investigation for Salt Interference and an Algorithm for Optimization. Journal of Proteome Research 09/2010; 9(11):5542-56., DOI:10.1021/pr1008256
  34. Chung-Mao Ou, Shih-Ren Lin, Han-Jia Lin, Ching-Wei Luo, Yee-Hsiung Chen: Exclusive Expression of a Membrane-Bound Spink3-Interacting Serine Protease-Like Protein TESPL in Mouse Testis. Journal of Cellular Biochemistry 06/2010; 110(3):620-9., DOI:10.1002/jcb.22571
  35. Cheng-Kang Chiang, Zusing Yang, Yang-Wei Lin, Wen-Tsen Chen, Han-Jia Lin, Huan-Tsung Chang: Detection of Proteins and Protein-Ligand Complexes Using HgTe Nanostructure Matrixes in Surface-Assisted Laser Desorption/Ionization Mass Spectrometry. Analytical Chemistry 06/2010; 82(11):4543-50., DOI:10.1021/ac100550c
  36. Huan-Chin Tseng, Han-Jia Lin, Jyh-Bing Tang, P.S. Sudhakar Gandhi, Wei-Chao Chang, Yee-Hsiung Chen: Identification of the Major TG(4) Cross-Linking Sites in the Androgen-Dependent SVS I Exclusively Expressed in Mouse Seminal Vesicle. Journal of Cellular Biochemistry 08/2009; 107(5):899-907., DOI:10.1002/jcb.22190
  37. Huan-Chin Tseng, Han-Jia Lin, P. S. Sudhakar Gandhi, Chia-Yih Wang, Yee-Hsiung Chen: Purification and identification of transglutaminase from mouse coagulating gland and its cross-linking activity among seminal vesicle secretion proteins. Journal of Chromatography B 11/2008; 876(2):198-202., DOI:10.1016/j.jchromb.2008.10.041
  38. Yee-Hsiung Chen, Huan-Chin Tseng, Han-Jia Lin, Chia-Yih Wang: An Interlocking Meshwork of Covalent Cross-Links between SVS I-III from the Mouse SeminalVesicle are involved in Copulatory Plug Formation. Biology of Reproduction 05/2008; 78(Suppl_1):220-220., DOI:10.1093/biolreprod/78.s1.220a
  39. Han-Jia Lin, Ching-Wei Luo, Chia-Yih Wang, Yee-Hsiung Chen: Epitope topology and removal of mouse acrosomal plasma membrane by P12-targeted immunoaggregation. Biochemical and Biophysical Research Communications 11/2006; 349(1):284-8., DOI:10.1016/j.bbrc.2006.08.045
  40. Han-Jia Lin, Chun-Hui Lin, Huan-Chin Tseng, Yee-Hsiung Chen: Detecting disulfide crosslinks of high-molecular weight complexes in mouse SVS proteins by diagonal electrophoresis. Analytical Biochemistry 06/2006; 352(2):296-8., DOI:10.1016/j.ab.2006.03.005
  41. HJ Lin, CH Lin, HC Tseng, YH Chen: Detecting disulfide crosslinks of high-molecular weight complexes in mouse SVS proteins by diagonal electrophoresis. Analytical Biochemistry 05/2006; 352(2):296-298., DOI:10.1016/j.sb.2006.03.005
  42. Han-Jia Lin, Chin-Mei Lee, Ching-Wei Luo, Yee-Hsiung Chen: Functional preservation of duplicated pair for RSVS III gene in the REST locus of rat 3q42. Biochemical and Biophysical Research Communications 02/2005; 326(2):355-63., DOI:10.1016/j.bbrc.2004.11.030
  43. Ching-Wei Luo, Han-Jia Lin, S C B Gopinath, Yee-Hsiung Chen: Distinction of Sperm-Binding Site and Reactive Site for Trypsin Inhibition on P12 Secreted from the Accessory Sex Glands of Male Mice. Biology of Reproduction 05/2004; 70(4):965-71., DOI:10.1095/biolreprod.103.020552
  44. Han-Jia Lin, Ching-Wei Luo, Yee-Hsiung Chen: Localization of the Transglutaminase Cross-linking Site in SVS III, a Novel Glycoprotein Secreted from Mouse Seminal Vesicle. Journal of Biological Chemistry 03/2002; 277(5):3632-9., DOI:10.1074/jbc.M107578200
  45. C W Luo, H J Lin, YH Chen: A Novel Heat-labile Phospholipid-binding Protein, SVS VII, in Mouse Seminal Vesicle as a Sperm Motility Enhancer. Journal of Biological Chemistry 04/2001; 276(10):6913-21., DOI:10.1074/jbc.M006954200
  46. S T Chu, H J Lin, H L Huang, YH Chen: The hydrophobic pocket of 24p3 protein from mouse uterine luminal fluid: Fatty acid and retinol binding activity and predicted structural similarity to lipocalins. Journal of Peptide Research 12/1998; 52(5):390-7., DOI:10.1111/j.1399-3011.1998.tb00663.x
  47. SIN‐TAK CHU, HAN‐JIA LIN, YEE‐HSIUNG CHEN: Complex formation between a formyl peptide and 24p3 protein with a blocked N-terminus of pyroglutamate. European Journal of Peptide Research 07/1997; 49(6):582-5., DOI:10.1111/j.1399-3011.1997.tb01166.x
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